META 2021, META'12

Font Size: 
Multiplexing and confinement in fluorescence correlation spectroscopy with an array of optical fibers.
Fiona Quinlan-Pluck, Quentin Wilmart, Emmanuel Fort, Neso Sojic, Sandrine Leveque-Fort, Samuel Gresillon

Last modified: 2011-11-01


We use an ordered array of etched optical fibers and an electron multiplying charge coupled device camera to create a multi-points observation platform for fluorescence correlation spectroscopy (FCS). The etched optical fibers improve the resolution of standard FCS and create well separated correlation volumes. The latter avoids the common cross talk problem of multiplexed FCS. Thus our set-up performs multiplexed FCS with precise localisation and confinement. Measurements on fluorescent beads are compared with standard FCS. Correlations show that, in this new set-up, both spatial resolution and confinement within the range of confocal microscopy.


FCS; near-field optical fibre; multiplexing resolution