META 2021, META'12

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Nucleic Acid Detection Using Plasmonic Coupling Interference (PCI) Nanoprobes
T. Vo-Dinh

Last modified: 2011-12-09


We have developed a novel label-free approach using surface-enhanced Raman scattering (SERS)-based plasmonic coupling interference (PCI) nanoprobes for nucleic acid detection. To induce a strong plasmonic coupling effect and SERS signal, a nano-network of silver nanoparticles having the Raman label located between adjacent nanoparticles is assembled by Raman-labeled nucleic acid duplexes. The PCI method then utilizes specific nucleic acid sequences of interest as competitor elements of the Raman-labeled DNA strands to interfere the formation of the nucleic acid-cross-linked nano-networks in a competitive binding process, resulting in a reduced SERS signal. We illustrate the potential of the PCI technique for biomedical applications by detecting single-nucleotide polymorphism (SNP) and microRNA sequences involved in breast cancers. The results of this study could lead to the development of nucleic acid diagnostic tools for biomedical diagnostics and biosensing applications using SERS detection.