META 2021, META'12

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Evaluation of SERS labeling of CD20 on CLL cells using optical microscopy and fluorescence flow cytometry
Christina MacLaughlin, Edward Parker, Chen Wang, Gilbert Walker

Last modified: 2011-12-05


Aims: To evaluate the chemical design and ability of surface-enhanced Raman scattering (SERS) gold nanoprobes to recognize cell surface receptors, probes are targeted to CD20 on cells using rituximab. Materials & Methods: SERS nanoparticles were prepared by adsorbing a Raman-active dye onto the surface of Au nanoparticles of different shapes, coating the particles with 5kDa polyethylene glycol (PEG) and conjugating rituximab to functional groups on PEG.  Targeting of CLL cells by the NPs was evaluated by dark field microscopy, Raman spectroscopy, and flow cytometry with competitive binding and fluorescence detection.  Results & Conclusion: Rituximab-SERS Au NPs selectively labeled CD20 molecules on the surface of patient derived B lymphocytes, as evaluated using multiple detection methods.  Evidence of CD20 clustering was observed.